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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Unidades Centrais. |
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Data corrente: |
19/03/2018 |
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Data da última atualização: |
20/03/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BIER, D.; TUTIJA, J. F.; PASQUATTI, T. N.; OLIVEIRA, T. L.; ARAUJO, F. R.; VERBISCK, N. V. |
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Afiliação: |
Daniele Bier, FAMEZ/Universidade Federal de Mato Grosso do Sul - UFMS; Juliane F. Tutija, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; Taynara N. Pasquatti, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; Tayná L. Oliveira, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; FLABIO RIBEIRO ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC. |
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Título: |
Identificação por espectrometria de massa MALDI-TOF de Salmonella spp. e Escherichia coli isolados de carcaças bovinas. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1373-1379, dezembro 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em inglês: MALDI-TOF mass spectrometry identification of Salmonella spp. and Escherichia coli isolated from bovine carcasses. |
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Conteúdo: |
The aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper confirmed. MALDI-TOF methodology allowed rapid Salmonella spp. and E. coli identity confirmation and may be used to detect these microrganisms within bacterial isolates from beef carcasses. MenosThe aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper... Mostrar Tudo |
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Palavras-Chave: |
MALDI–TOF; Slaughterhouse. |
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Thesagro: |
Escherichia Coli; Salmonella spp. |
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Thesaurus Nal: |
Beef carcasses; Enterobacteriales; Mass spectrometry. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174160/1/Identificacao-por-espectrometria-de-massa-MALDI-TOF.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174156/1/Identificacao-por-espectrometria-de-massa-MALDI-TOF.pdf
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Marc: |
LEADER 02676naa a2200277 a 4500
001 2089384
005 2018-03-20
008 2017 bl uuuu u00u1 u #d
100 1 $aBIER, D.
245 $aIdentificação por espectrometria de massa MALDI-TOF de Salmonella spp. e Escherichia coli isolados de carcaças bovinas.$h[electronic resource]
260 $c2017
500 $aTítulo em inglês: MALDI-TOF mass spectrometry identification of Salmonella spp. and Escherichia coli isolated from bovine carcasses.
520 $aThe aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper confirmed. MALDI-TOF methodology allowed rapid Salmonella spp. and E. coli identity confirmation and may be used to detect these microrganisms within bacterial isolates from beef carcasses.
650 $aBeef carcasses
650 $aEnterobacteriales
650 $aMass spectrometry
650 $aEscherichia Coli
650 $aSalmonella spp
653 $aMALDI–TOF
653 $aSlaughterhouse
700 1 $aTUTIJA, J. F.
700 1 $aPASQUATTI, T. N.
700 1 $aOLIVEIRA, T. L.
700 1 $aARAUJO, F. R.
700 1 $aVERBISCK, N. V.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 12, p. 1373-1379, dezembro 2017.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 4 | |
| 1. |  | OLIVEIRA FILHO, J. G. de; BERTOLO, M. R. V.; BRITO, S. C.; MALAFATTI, J. O. D.; BERTAZZO, G. B.; COLACIQUE, M. N.; PARIS, E. C.; BOGUSZ JUNIOR, S.; FERREIRA, M. D. Recent advances in the application of nanotechnology to reduce fruit and vegetable losses during post-harvest. Brazilian Journal of Physics, v. 52, 126, 2022. 11 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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| 2. | | OLDONI, F. C. A.; FLORENCIO, C.; BERTAZZO, G. B.; GRIZOTTO, P. A.; BOGUSZ JUNIOR, S.; CARNEIRO, R. L.; COLNAGO, L. A.; FERREIRA, M. D. Fruit quality parameters and volatile compounds from ?Palmer? mangoes with internal breakdown. Food Chemistry, v. 388, 132902, 2022. 9 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 3. | | BERTAZZO, G. B.; OLDONI, F. C. A.; FLORENCIO, C.; BOGUSZ JUNIOR, S.; FERREIRA, M. D. Avaliação de compostos voláteis em mamão (Carica papaya L). In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 12., 2020, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2020. Editores técnicos: Cristiane Sanchez Farinas, Daniel Souza Corrêa, José Manoel Marconcini, Maria Fernanda Berlingieri Durigan, Paulo Sérgio de Paula Herrmann Junior. Embrapa Instrumentação. Documentos, 71.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | BERTAZZO, G. B.; GRIZOTTO, P. A.; OLDONI, F. C. A.; FLORENCIO, C.; BOGUSZ JUNIOR, S.; FERREIRA, M. D. Avaliação dos compostos voláteis em manga (Mangifera indica L). In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 11., 2019, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2019. Editores técnicos: Alexandre Berndt, Ana Rita de Araujo Nogueira, Lea Chapaval, Marcelo Mattos Cavallari, Manuel Antonio Chagas Jacinto. 50 Embrapa Pecuária Sudeste. Documentos, 134. 1980-6841| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 4 | |
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